目的 研究红象牙芒果核仁抗炎的活性成分。方法 采用硅胶柱色谱、Sephadex LH-20柱色谱、ODS柱色谱等分离手段,根据波谱学数据和理化性质鉴定化合物的结构;通过研究化合物对脂多糖(LPS)诱导的小鼠RAW264.7巨噬细胞产生NO浓度的影响,评价化合物的抗炎活性。 结果 从红象牙芒果核仁的正丁醇部位得到8个化合物,分别为4-O-乙基没食子酸(1)、没食子酸(2)、柠檬酸(3)、五-O-没食子酰葡萄糖(4)、三-O-没食子酰葡萄糖(5)、金丝桃苷(6)、槲皮素-3-O-鼠李糖苷(7)和芒果苷(8)。化合物1、2、4~8对LPS诱导的RAW264.7巨噬细胞NO的释放具有抑制作用,尤其是化合物6和8抑制作用最强,IC50值分别为16.5、 19.5μmol·L-1。结论 化合物1、3~8为首次从红象牙芒果核仁中分离得到;化合物1、2、4~8为红象牙芒果核仁抗炎活性成分,化合物6和8的抗炎活性最强。
Abstract
OBJECTIVE To study the anti-inflammatory active constituents of Mangifera indica L. seed kernel. METHODS The compounds were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography and ODS column chromatography. Their structures were elucidated by spectral analyses and physicochemical properties. The anti-inflammatory activities of selected isolated compounds were evaluated as inhibitory activities against lipopolysaccharide(LPS)-induced nitric oxide(NO) production in RAW264.7 cell lines by Griess reaction. RESULTS Eight compounds were obtained from n-butanol fraction of Mangifera indica L. seed kernel,whose structures were elucidated as 4-O-ethylgallic acid(1), gallic acid(2), citric acid(3), 1,2,3,4,6-penta-O-galloylglucose(4),1,3,6-tri-O-galloylglucose(5), hyperoside(6), quercetin-3-O-rhamnopyranoside(7), and mangiferin(8). Compounds 1, 2 and 4-8 exhibited potent inhibitory effect on NO production in LPS-induced macrophages, especially compounds 6 and 8 showed the best inhibitory activity with IC50 values of 16.5 and 19.5 μmol·L-1, respectively. CONCLUSION Compounds 1 and 3-8 were obtained from Mangifera indica L. seed kernel for the first time. Compounds 1, 2 and 4-8 are the anti-inflammatory active constituents of Mangifera indica L. seed kernel. Compounds 6 and 8 are the most potent anti-inflammatory active constituents.
关键词
红象牙芒果核仁 /
抗炎 /
4-O-乙基没食子酸 /
五-O-没食子酰葡萄糖 /
三-O-没食子酰葡萄糖
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Key words
Mangifera indica L. seed kernel /
anti-inflammatory /
4-O-ethylgallic acid /
1,2,3,4,6-penta-O-galloylglucose /
1,3,6-tri-O-galloylglucose
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中图分类号：
R284
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参考文献
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脚注
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基金
广西中药药效研究重点实验室开放基金资助项目(13-051-06-K5);四川省科技支撑计划资助项目(14ZC0043)
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